Nikita Oskolkov

https://www.etis.ee/CV/Nikita_Oskolkov/est
21.02.1979
+14436427192
nikita.oskolkov@gmail.com

Teenistuskäik

Töökohad ja ametid
15.11.2011–01.01.2016   
Johns Hopkins University, Järeldoktor (1,00)
01.09.2010–20.11.2011   
Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut, teadur (1,00)
10.02.2009–31.08.2010   
Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut, teadur (0,50)
01.03.2008–31.08.2010   
Cepep Eesti OÜ, Teadur (1,00)
11.09.2006–28.02.2008   
Narva Linnavalitsuse Arhitektuuri- ja Linnaplaneerimise Amet, Üldplaneeringute projektijuht (1,00)
2003–2006   
Celecure AS, peptiidsünteesi ja antikehade tootmise projektijuht (1,00)
01.01.2003–31.12.2003   
OÜ Luunja Labor, projektijuht
2002–2003   
Asper Biotech AS, teadur (0,50)
 
 
Haridustee
1997–2011   
Tartu Ülikool
1993–1997   
Pähklimäe Gümnaasium
 
 
Teadusorganisatsiooniline ja -administratiivne tegevus
2012−...   
International Society for Magnetic Resonance in Medicine (ISMRM)
2008−...   
Euroopa Peptiidide Ühingu liige
2005−...   
Eesti Biokeemia Ühingu liige
 
 
Teenistuskäigu lisainfo
Väliskogemus:
1999 Külalisteadur (TEMPUS scholarship), 1 kuu, Department of Mathematics, Hypermedia Laboratory, Tampere University of Technology, Tampere, Soome
2000 Külalisteadur (Swedish Institute scholarship), 9 kuud, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Rootsi
2001 Külalisteadur (ERASMUS scholarship), 3 kuud, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Rootsi
2001 Külalisteadur (scholarship from Swedish Biological Polymer Fund), 1 kuu, Department of Medical Biochemistry and Microbiology, Uppsala University, Swedish
;

Kvalifikatsioon

Teaduskraadid
Nikita Oskolkov, doktorikraad, 2011, (juh) Ülo Langel, A novel strategy for peptide-mediated cellular delivery and induction of endosomal escape (Peptiidide rakkudesse sisenemise ja rakusisestest endotsütootilistest vesiikulitest vabanemise uudne strateegia), Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Keemia Instituut.
Nikita Oskolkov, magistrikraad, 2005, (juh) Jaak Järv, Substrate Specificity Of Protein Kinase A In Reaction With Protein Substrates, Tartu Ülikool, Füüsika-keemiateaduskond.
 
 
Teadustöö põhisuunad
VALDKOND: 4. Loodusteadused ja tehnika; 4.16. Biotehnoloogia (loodusteadused ja tehnika); CERCS ERIALA: T360 Biokeemiatehnoloogia ; PÕHISUUND: Rakku tungivad peptiidid ja nende rakendamine geeniteraapias.Oligonukleotiidide ja plasmiidide rakku kohale toimetamine

Publikatsioonid

Klass
Aasta
Publikatsioon
 
1.1.
2013
Pärn, K.; Viru, L.; Lehto, T.; Oskolkov, N.; Langel, Ü.; Merits, A. (2013). Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penetrating Peptide Based Reagent PepFect6. PLoS ONE, 8 (7), e69659.10.1371/journal.pone.0069659.
1.1.
2013
Arukuusk, P.; Pärnaste, L.; Oskolkov, N.; Copolovici, D. M.; Margus, H.; Padari, K.; Möll, K.; Maslovskaja, J.; Tegova, R.; Kivi, G.; Tover, A.; Pooga, M.; Ustav, M.; Langel, Ü. (2013). New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids. Biochimica et Biophysica Acta - Biomembranes, 1828 (5), 1365−1373.10.1016/j.bbamem.2013.01.011.
1.1.
2013
Eriste, Elo; Kurrikoff, Kaido; Suhorutšenko, Julia; Oskolkov, Nikita; Copolovici, Dana Maria; Jones, Sarah; Howl, John; Laakkonen, Pirjo; Langel, Ülo (2013). Peptide-based glioma-targeted drug delivery vector gHoPe2. Bioconjugate Chemistry, 24 (3), 305−313.10.1021/bc300370w.
1.1.
2012
Lorents, A.; Oskolkov, N.; Langel, Ü.; Hällbrink, M.; Pooga, M. (2012). Cell-penetrating peptides split into two groups based on the modulation of the intracellular calcium concentration. Journal of Biological Chemistry, 287 (20), 16880−16889.10.1074/jbc.M111.318063.
1.1.
2011
Suhorutšenko, J.; Oskolkov, N.; Arukuusk, P.; Kurrikoff, K.; Eriste, E.; Copolovici, D.-M.; Langel, Ü. (2011). Cell-Penetrating Peptides, PepFects, Show No Evidence of Toxicity and Immunogenicity In Vitro and In Vivo. Bioconjugate Chemistry, 22 (11), 2255−2262.dx.doi.org/10.1021/bc200293d.
1.1.
2011
Oskolkov, N.; Arukuusk, P.; Copolovici, D.-M.; Lindberg, S.; Margus, H.; Padari, K.; Pooga, M.; Langel, Ü. (2011). NickFects, Phosphorylated Derivatives of Transportan 10 for Cellular Delivery of Oligonucleotides. International Journal of Peptide Research and Therapeutics, 17 (2), 147−157.10.1007/s10989-011-9252-1.
1.1.
2011
Lehto, T.; Simonson, O.E.; Mäger, I.; Ezzat, K.; Sork, H.; Copolovici, D.-M.; Viola, J.R.; Zaghloul, E.M.; Lundin, P.; Moreno, P.M.D.; Mäe, M.; Oskolkov, N.; Suhorutšenko, J.; Smith, C.E.; EL Andaloussi, S. (2011). A peptide-based vector for efficient gene transfer in vitro and in vivo. Molecular therapy, 19 (8), 1457−1467.10.1038/mt.2011.10.
1.1.
2011
El Andaloussi, S; Lehto, T; Mager, I; Rosenthal-Aizman, K; Oprea, I.I; Simonson, O.E; Sork, H; Ezzat, K; Copolovici, D.M; Kurrikoff, K; Viola, J.R; Zaghloul, E.M; Sillard, R; Johansson, H. J; Hassane, F.S; Guterstam, P.; Suhorutsenko, J.; Moreno, P. M. D.; Oskolkov, N; Halldin, J ... Langel, U. (2011). Design of a peptide-based vector, PepFect6, for efficient delivery of siRNA in cell culture and systemically in vivo. Nucleic Acids Research, 39 (9), 3972−3987.10.1093/nar/gkq1299.
1.1.
2010
Lehto, T.; Abes, R.; Oskolkov, N.; SuhorutÅ¡enko, J.; Copolovici, D.M.; Mager, I.; Viola, J.R.; Simonson, O.E.; Ezzat, K.; Guterstam, P. (2010). Delivery of nucleic acids with a stearylated (RxR)(4) peptide using a non-covalent co-incubation strategy. Journal of Controlled Release, 141 (1), 42−51.10.1016/j.jconrel.2009.08.028.
1.1.
2010
Lehto, T.; Abes, R.; Oskolkov, N.; Suhorutśenko, J.; Copolovici, D.-M.; Mäger, I.; Viola, J.R.; Simonsson, O.; Guterstam, P.; Eriste, E.; Smith, C.I. E.; Lebleu, B.; El-Andaloussi, S.; Langel, Ü. (2010). Delivery of nucleic acids with a stearylated (RxR)4 peptide using a non-covalent co-incubation strategy. Journal of Controlled Release, 141 (1), 42−51.10.1016/j.jconrel.2009.08.028.
5.1.
2010
Padari, K.; Juks, C.; Oskolkov, N.; Raid, R.; Langel, Ü.; Pooga, M. (2010). Cellular delivery of oligonucleotides by PepFect. In: (1092−1093).. 10.1016/j.drudis.2010.09.386.
5.1.
2010
Lorents, A.; Oskolkov, N.; Tõnissoo, T.; Langel, Ü.; Hällbrink, M.; Pooga, M. (2010). Amphipathic CPPs upregulate Ca in cells’ cytosol and induce lysosomal exocytosis. In: (A34).. 10.1016/j.drudis.2010.09.385.
5.1.
2010
Suhorutsenko, J.; Copolovici, D. M.; Eriste, E.; Oskolkov, N.; Arukuusk, P.; Langel, Ü. (2010). New cell-penetrating peptides induce no inflammatory response in antigen-presenting cell lines. Scandinavian Journal of Immunology, 71: 39th meeting of Scandinavian Society for Immunology jointly with the Baltic Immunologycal Society; Tallinn, Estonia; 2-5.06.2010. Blackwell Publishing Ltd, 508−509. (474-548; 6).10.1111/j.1365-3083.2010.02392.x.
1.1.
2009
Mäe, M.; El-Andaloussi, S.; Lundin, P.; Oskolkov, N.; Johansson, H.J.; Guterstam, P.; Langel, Ü. (2009). A stearylated CPP for delivery of splice correcting oligonucleotides using a noncovalent co-incubation strategy. Journal of Controlled Release, 134 (3), 221−227.
5.1.
2006
Faustova, I.; Oskolkov, N.; Järv, J. (2006). Effect of Net Charge of the Regulatory Domain on Catalytic Properties of L-Pyruvate Kinase. FEBS Journal, 273: 31st FEBS Congress; Istanbul, Turkey; 24-29 June, 2006. 342. (S1).
1.1.
2005
Loog, M.; Oskolkov, N.; O'Farrell, F.; Ek, P.; Jarv, J. (2005). Comparison of cAMP-dependent protein kinase substrate specificity in reaction with proteins and synthetic peptides. Biochimica et Biophysica Acta - Proteins and Proteomics, 1747 (2), 261−266.
1.1.
2005
Loog, M.; Ek, B.; Oskolkov, N.; Narvanen, A.; Jarv, J.; Ek, P. (2005). Screening for the optimal specificity profile of protein kinase C using electrospray mass-spectrometry. Journal of Biomolecular Screening, 10 (4), 320−328.
5.1.
2005
Faustova, I.; Kuznetsov, A.; Oslolkov, N.; Jarv, J. (2005). Kinetic properties of some mutants of l-pyruvate kinase. FEBS Journal, 272. Blackwell Publishing, 369−370.
1.2.
2003
Kuznetsov, A.; Oskolkov, N.; Hansen, M.; Järv, J. (2003). Steady-state kinetic analysis of protein kinase A interaction with peptide and ATP. Proceedings of the Estonian Academy of Sciences. Chemistry, 52 (4), 165−177.

Tööstusomand

18.04.2019

Nikita Oskolkov

https://www.etis.ee/CV/Nikita_Oskolkov/eng
21.02.1979
+14436427192
nikita.oskolkov@gmail.com

Career

Institution(s) and position(s)
15.11.2011–01.01.2016   
Johns Hopkins University, Postdoctoral Fellow (1,00)
01.09.2010–20.11.2011   
University of Tartu, Faculty of Science and Technology (old), Institute of Technology, University of Tartu, Research Fellow (1,00)
10.02.2009–31.08.2010   
University of Tartu, Faculty of Science and Technology (old), Institute of Technology, University of Tartu, Research Fellow (0,50)
01.03.2008–31.08.2010   
Cepep Eesti OÜ, Researcher (1,00)
11.09.2006–28.02.2008   
Department of Architecture and City Planning, Project manager (1,00)
2003–2006   
Celecure, Project manager (Peptide synthesis and antibody production) (1,00)
01.01.2003–31.12.2003   
OÜ Luunja Labor, project manager
2002–2003   
Asper Biotech, scientist (0,50)
 
 
Education
1997–2011   
Tartu University
1993–1997   
Pähklimäe Gymnasium
 
 
R&D related managerial and administrative work
2012−...   
Member of International Society for Magnetic Resonance in Medicine (ISMRM)
2008−...   
Member of European Peptide Society
2005−...   
Member of Estonian Biochemical Society
 
 
Additional career information
Foreign experience:
1999 Guest scientist awarded with TEMPUS scholarship for 1 month in Department of Mathematics, Hypermedia Laboratory at Tampere University of Technology, Tampere, Finland
2000 Guest scientist awarded with Swedish Institute scholarship for 9 months in Department of Medical Biochemistry and Microbiology at Uppsala University, Uppsala, Sweden
2001 Guest scientist awarded with ERASMUS scholarship for 3 months in Department of Medical Biochemistry and Microbiology at Uppsala University, Uppsala, Sweden
2001 Guest scientist awarded with scholarship from Swedish Biological Polymer Fund for 1 month in Department of Medical Biochemistry and Microbiology at Uppsala University, Sweden
;

Qualifications

Academic degrees
Nikita Oskolkov, Doctor's Degree, 2011, (sup) Ülo Langel, A novel strategy for peptide-mediated cellular delivery and induction of endosomal escape, University of Tartu, Faculty of Science and Technology (old), Institute of Chemistry, Tartu University.
Nikita Oskolkov, Master's Diploma, 2005, (sup) Jaak Järv, Substrate Specificity Of Protein Kinase A In Reaction With Protein Substrates, University of Tartu, Faculty of Physics and Chemistry.
 
 
Field of research
FIELD OF RESEARCH: 4. Natural Sciences and Engineering; 4.16. Biotechnology relating to Natural Sciences and Engineering; CERCS SPECIALITY: T360 Biochemical technology ; SPECIALITY: Cell-penetrating peptides and their application in gene therapy. Oligonucleotides and plasmids intracellular delivery

Publications

Category
Year
Publication
 
1.1.
2013
Pärn, K.; Viru, L.; Lehto, T.; Oskolkov, N.; Langel, Ü.; Merits, A. (2013). Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penetrating Peptide Based Reagent PepFect6. PLoS ONE, 8 (7), e69659.10.1371/journal.pone.0069659.
1.1.
2013
Arukuusk, P.; Pärnaste, L.; Oskolkov, N.; Copolovici, D. M.; Margus, H.; Padari, K.; Möll, K.; Maslovskaja, J.; Tegova, R.; Kivi, G.; Tover, A.; Pooga, M.; Ustav, M.; Langel, Ü. (2013). New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids. Biochimica et Biophysica Acta - Biomembranes, 1828 (5), 1365−1373.10.1016/j.bbamem.2013.01.011.
1.1.
2013
Eriste, Elo; Kurrikoff, Kaido; Suhorutšenko, Julia; Oskolkov, Nikita; Copolovici, Dana Maria; Jones, Sarah; Howl, John; Laakkonen, Pirjo; Langel, Ülo (2013). Peptide-based glioma-targeted drug delivery vector gHoPe2. Bioconjugate Chemistry, 24 (3), 305−313.10.1021/bc300370w.
1.1.
2012
Lorents, A.; Oskolkov, N.; Langel, Ü.; Hällbrink, M.; Pooga, M. (2012). Cell-penetrating peptides split into two groups based on the modulation of the intracellular calcium concentration. Journal of Biological Chemistry, 287 (20), 16880−16889.10.1074/jbc.M111.318063.
1.1.
2011
Suhorutšenko, J.; Oskolkov, N.; Arukuusk, P.; Kurrikoff, K.; Eriste, E.; Copolovici, D.-M.; Langel, Ü. (2011). Cell-Penetrating Peptides, PepFects, Show No Evidence of Toxicity and Immunogenicity In Vitro and In Vivo. Bioconjugate Chemistry, 22 (11), 2255−2262.dx.doi.org/10.1021/bc200293d.
1.1.
2011
Oskolkov, N.; Arukuusk, P.; Copolovici, D.-M.; Lindberg, S.; Margus, H.; Padari, K.; Pooga, M.; Langel, Ü. (2011). NickFects, Phosphorylated Derivatives of Transportan 10 for Cellular Delivery of Oligonucleotides. International Journal of Peptide Research and Therapeutics, 17 (2), 147−157.10.1007/s10989-011-9252-1.
1.1.
2011
Lehto, T.; Simonson, O.E.; Mäger, I.; Ezzat, K.; Sork, H.; Copolovici, D.-M.; Viola, J.R.; Zaghloul, E.M.; Lundin, P.; Moreno, P.M.D.; Mäe, M.; Oskolkov, N.; Suhorutšenko, J.; Smith, C.E.; EL Andaloussi, S. (2011). A peptide-based vector for efficient gene transfer in vitro and in vivo. Molecular therapy, 19 (8), 1457−1467.10.1038/mt.2011.10.
1.1.
2011
El Andaloussi, S; Lehto, T; Mager, I; Rosenthal-Aizman, K; Oprea, I.I; Simonson, O.E; Sork, H; Ezzat, K; Copolovici, D.M; Kurrikoff, K; Viola, J.R; Zaghloul, E.M; Sillard, R; Johansson, H. J; Hassane, F.S; Guterstam, P.; Suhorutsenko, J.; Moreno, P. M. D.; Oskolkov, N; Halldin, J ... Langel, U. (2011). Design of a peptide-based vector, PepFect6, for efficient delivery of siRNA in cell culture and systemically in vivo. Nucleic Acids Research, 39 (9), 3972−3987.10.1093/nar/gkq1299.
1.1.
2010
Lehto, T.; Abes, R.; Oskolkov, N.; SuhorutÅ¡enko, J.; Copolovici, D.M.; Mager, I.; Viola, J.R.; Simonson, O.E.; Ezzat, K.; Guterstam, P. (2010). Delivery of nucleic acids with a stearylated (RxR)(4) peptide using a non-covalent co-incubation strategy. Journal of Controlled Release, 141 (1), 42−51.10.1016/j.jconrel.2009.08.028.
1.1.
2010
Lehto, T.; Abes, R.; Oskolkov, N.; Suhorutśenko, J.; Copolovici, D.-M.; Mäger, I.; Viola, J.R.; Simonsson, O.; Guterstam, P.; Eriste, E.; Smith, C.I. E.; Lebleu, B.; El-Andaloussi, S.; Langel, Ü. (2010). Delivery of nucleic acids with a stearylated (RxR)4 peptide using a non-covalent co-incubation strategy. Journal of Controlled Release, 141 (1), 42−51.10.1016/j.jconrel.2009.08.028.
5.1.
2010
Padari, K.; Juks, C.; Oskolkov, N.; Raid, R.; Langel, Ü.; Pooga, M. (2010). Cellular delivery of oligonucleotides by PepFect. In: (1092−1093).. 10.1016/j.drudis.2010.09.386.
5.1.
2010
Lorents, A.; Oskolkov, N.; Tõnissoo, T.; Langel, Ü.; Hällbrink, M.; Pooga, M. (2010). Amphipathic CPPs upregulate Ca in cells’ cytosol and induce lysosomal exocytosis. In: (A34).. 10.1016/j.drudis.2010.09.385.
5.1.
2010
Suhorutsenko, J.; Copolovici, D. M.; Eriste, E.; Oskolkov, N.; Arukuusk, P.; Langel, Ü. (2010). New cell-penetrating peptides induce no inflammatory response in antigen-presenting cell lines. Scandinavian Journal of Immunology, 71: 39th meeting of Scandinavian Society for Immunology jointly with the Baltic Immunologycal Society; Tallinn, Estonia; 2-5.06.2010. Blackwell Publishing Ltd, 508−509. (474-548; 6).10.1111/j.1365-3083.2010.02392.x.
1.1.
2009
Mäe, M.; El-Andaloussi, S.; Lundin, P.; Oskolkov, N.; Johansson, H.J.; Guterstam, P.; Langel, Ü. (2009). A stearylated CPP for delivery of splice correcting oligonucleotides using a noncovalent co-incubation strategy. Journal of Controlled Release, 134 (3), 221−227.
5.1.
2006
Faustova, I.; Oskolkov, N.; Järv, J. (2006). Effect of Net Charge of the Regulatory Domain on Catalytic Properties of L-Pyruvate Kinase. FEBS Journal, 273: 31st FEBS Congress; Istanbul, Turkey; 24-29 June, 2006. 342. (S1).
1.1.
2005
Loog, M.; Oskolkov, N.; O'Farrell, F.; Ek, P.; Jarv, J. (2005). Comparison of cAMP-dependent protein kinase substrate specificity in reaction with proteins and synthetic peptides. Biochimica et Biophysica Acta - Proteins and Proteomics, 1747 (2), 261−266.
1.1.
2005
Loog, M.; Ek, B.; Oskolkov, N.; Narvanen, A.; Jarv, J.; Ek, P. (2005). Screening for the optimal specificity profile of protein kinase C using electrospray mass-spectrometry. Journal of Biomolecular Screening, 10 (4), 320−328.
5.1.
2005
Faustova, I.; Kuznetsov, A.; Oslolkov, N.; Jarv, J. (2005). Kinetic properties of some mutants of l-pyruvate kinase. FEBS Journal, 272. Blackwell Publishing, 369−370.
1.2.
2003
Kuznetsov, A.; Oskolkov, N.; Hansen, M.; Järv, J. (2003). Steady-state kinetic analysis of protein kinase A interaction with peptide and ATP. Proceedings of the Estonian Academy of Sciences. Chemistry, 52 (4), 165−177.

Industrial property

18.04.2019
Detailne otsing
  • Leitud 3 kirjet
ProgrammLiikNumberNimiProjekti algusProjekti lõppVastutav täitjaAsutusFinantseerijapoolne rahastamine
SFSF0180027s08Koespetsiifilised transportvektorid01.01.200831.12.2013Ülo LangelTartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut600 272,61 EUR
ETFETF8679Süsteemselt aktiivsete galaniini retseptori ligandide ratsionaalne disain01.01.201131.12.2014Kaido KurrikoffTartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut28 800,00 EUR
SFSF0180064s08Uute biomimeetikute kui võimalike bioaktiivsete materjalide konstrueerimine, süntees ja testimine01.01.200831.12.2013Jaak JärvTartu Ülikool, Füüsika-keemiateaduskond; Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Keemia Instituut; Tartu Ülikool, Loodus- ja tehnoloogiateaduskond988 870,12 EUR