See veebileht kasutab küpsiseid kasutaja sessiooni andmete hoidmiseks. Veebilehe kasutamisega nõustute ETISe kasutustingimustega. Loe rohkem
Olen nõus
20.02.1987
+420 731981177
+420 5 4949 8147
olga.jasnovidova@gmail.com

Teenistuskäik

Töökohad ja ametid
01.01.2010–...   
Masaryk University, Central European Institute of Technology, Brno, Czech Republic, spetsialist
2007–2010   
Quattromed HTI Laborid OÜ, laborant (0,50)
29.05.2006–03.09.2006   
Tartu Ülikool, Bioloogia-geograafiateaduskond, Zooloogia ja hüdrobioloogia instituut, laborant (0,20)
 
 
Haridustee
2010–...   
Masaryk University, CEITEC, Tsehhi, doktorantuur
2008–2010   
TÜ Ülikool,molekullar- ja rakubioloogia, magistriõpe
2005–2008   
TÜ Ülikool, bioloogia bakalauruseõpe
2001–2005   
TÜ Teaduskool, keemia
1993–2005   
Tallinna 53. Keskkool

Kvalifikatsioon

 
 
Teadustöö põhisuunad
VALDKOND: 4. Loodusteadused ja tehnika; 4.16. Biotehnoloogia (loodusteadused ja tehnika); CERCS ERIALA: T360 Biokeemiatehnoloogia ; PÕHISUUND: Struktuur bioloogia, Struktuur bioloogia
 
 
Lisainfo
Oskused
Medical diagnostics using PCR: viral and bacterial DNA purification from patient samples, PCR, agarose gel electrophoresis, validation of results and quality control.
Basic molecular cloning skills: design and performance of cloning; DNA amplification from plasmid, genomic and cDNA; plasmid DNA, RNA and cDNA isolation; site-directed mutagenesis; design of sequences for gene synthesis.
Model organisms: cultivation of E.coli, budding and fission yeast, HEK293 and HeLa cells.
Protein purification: optimization of protein expression in bacteria; design of large-scale protein purifications using ion-exchange, affinity and size-exclusion chromatography. Operation of AKTA FPLC machines.
Specific protein labeling: isotopic labeling of proteins with 15N, 13C and 2H for NMR; attachment of paramagnetic compounds; incorporation of unnatural amino acids.
Interaction studies: Study of protein-protein interactions using Small-angle X-ray scattering (SAXS), immuno-blotting, pull-down assay, isothermal titration calorimetry (ITC) and fluorescence anisotropy (FA).
Solution NMR: 1D, 2D and 3D NMR for studies of protein, structure, dynamics and interactions on Brucker Avance systems: preparation of protein samples; measurement of basic NMR experiments for proteins; NMR titrations; evaluation of NMR titration data and spin relaxation experiments; protein backbone assignment.
5.04.2018
20.02.1987
+420 731981177
+420 5 4949 8147
olga.jasnovidova@gmail.com

Career

Institution and occupation
01.01.2010–...   
Masaryk University, Central European Institute of Technology, Brno, Czech Republic, specialist
2007–2010   
Quattromed HTI Laborid OÜ, Laboratory technician, molecular diagnostics, quality control (0,50)
29.05.2006–03.09.2006   
University of Tartu, Faculty of Biology and Geography, Institute of Zoology and Hydrobiology, Laboratory Assistant (0,20)
 
 
Education
2010–...   
Masaryk University, CEITEC, Czech Republic, PhD study in structural biology
2008–2010   
University of Tartu, M.Sc. in molecular and cell biology
2005–2008   
University of Tartu, B.Sc. in biology
2001–2005   
The Science School of University of Tartu, Chemistry
1993–2005   
Tallinn 53rd Secondary School

Qualifications

 
 
Field of research
FIELD OF RESEARCH: 4. Natural Sciences and Engineering; 4.16. Biotechnology relating to Natural Sciences and Engineering; CERCS SPECIALTY: T360 Biochemical technology ; SPECIALITY: Structure biology, NMR, Structural biology
 
 
Additional information
Practical skills
Medical diagnostics using PCR: viral and bacterial DNA purification from patient samples, PCR, agarose gel electrophoresis, validation of results and quality control.
Basic molecular cloning skills: design and performance of cloning; DNA amplification from plasmid, genomic and cDNA; plasmid DNA, RNA and cDNA isolation; site-directed mutagenesis; design of sequences for gene synthesis.
Model organisms: cultivation of E.coli, budding and fission yeast, HEK293 and HeLa cells.
Protein purification: optimization of protein expression in bacteria; design of large-scale protein purifications using ion-exchange, affinity and size-exclusion chromatography. Operation of AKTA FPLC machines.
Specific protein labeling: isotopic labeling of proteins with 15N, 13C and 2H for NMR; attachment of paramagnetic compounds; incorporation of unnatural amino acids.
Interaction studies: Study of protein-protein interactions using Small-angle X-ray scattering (SAXS), immuno-blotting, pull-down assay, isothermal titration calorimetry (ITC) and fluorescence anisotropy (FA).
Solution NMR: 1D, 2D and 3D NMR for studies of protein, structure, dynamics and interactions on Brucker Avance systems: preparation of protein samples; measurement of basic NMR experiments for proteins; NMR titrations; evaluation of NMR titration data and spin relaxation experiments; protein backbone assignment.
5.04.2018