Eesti Teadusinfosüsteem

Patentne leiutis: Restriktaas vahendatud tagmenteerimine ja kromatiini immuunosadestamise sekveneerimine (RAT-CHiP); Omanikud: Tartu Ülikool, Loodus- ja täppisteaduste valdkond, molekulaar- ja rakubioloogia instituut, Tervisetehnoloogiate Arenduskeskus AS (endine Reproduktiivmeditsiini TAK AS) ; Autorid: Tõnis Org, Andres Salumets, Ants Kurg; Prioriteedi number: 1751281-5; Prioriteedi kuupäev: 16.10.2017.

Patentne leiutis

Restriktaas vahendatud tagmenteerimine ja kromatiini immuunosadestamise sekveneerimine (RAT-CHiP)

Restrictase Assisted Tagmentation Chromatin Immunoprecipitation sequencing (RAT-CHIP-seq)

A library of DNA molecules is prepared from a chromatin sample by enzymatic digestion of the chromatin sample. The resulting chromatin fragments are immobilized onto a support and tagged in a tagmentation process using a transposase. The DNA molecules of the tagged chromatin fragments immobilized onto the support are amplified using forward and reverse amplification primers to form the library of amplified DNA molecules. The library preparation requires only very minute starting materials and is thereby useful in applications with limited starting material, such as highly pure cell populations or early developmental stages of mammalian preimplantation embryo.

16.10.2017

1751281-5

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