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Paralleelne praimerekstensioon nukleiinhapete järjestuse analüüsil
Patentne leiutis: Paralleelne praimerekstensioon nukleiinhapete järjestuse analüüsil; Omanikud: PHARMACIA BIOTECH AB , GE HEALTHCARE BIO-SCIENCES AB (uus omanik alates 21.02.06); Autorid: Andres Metspalu, CASKEY C THOMAS, SHUMAKER JOHN; Prioriteedi number: SE9302152-5; Prioriteedi kuupäev: 22.06.1993.
Patentne leiutis
Paralleelne praimerekstensioon nukleiinhapete järjestuse analüüsil
Parallel primer extension approach to nucleic acid sequence analysis
A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end therof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.
  • PHARMACIA BIOTECH AB
  • GE HEALTHCARE BIO-SCIENCES AB (uus omanik alates 21.02.06)
Prioriteeditaotlus
22.06.1993
SE9302152-5
Rahvusvaheline (IPC)
C12Q1/68; C07H21/02; C12P19/34; C07H21/00; C12P19/00; C12Q1/68;
Rootsi Patendiamet
Rootsi Kuningriik
Kaitstud
Järgnevad taotlused (1)
KuupäevNumberPatendiametRiikURLFailStaatus
22.06.1994PCT/US94/07086USA patendiamet andis sisse PCT taotluseAmeerika Ühendriigid,Austria Vabariik,Austraalia,Kanada,Jaapan,Hispaania Kuningriik,Saksamaa LiitvabariikKaitstud