Piret Arukuusk

https://www.etis.ee/CV/Piret_Arukuusk/est
piret.arukuusk@ut.ee

Teenistuskäik

Töökohad ja ametid
01.01.2016–...   
Tartu Ülikool, Loodus- ja täppisteaduste valdkond, tehnoloogiainstituut, peptiidikeemia teadur (1,00)
15.11.2013–31.07.2015   
Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut, molekulaarse biotehnoloogia teadur (1,00)
01.09.2009–14.11.2013   
Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut, spetsialist (1,00)
 
 
Haridustee
2011–2013   
Tartu Ülikool, doktorant

Kvalifikatsioon

Teaduskraadid
Piret Arukuusk, doktorikraad, 2013, (juh) Ülo Langel, NickFects - novel cell-penetrating peptides. Design and uptake mechanism (NcikFectid - uued rakku sisenevad peptiidid. Disain ja sisenemismehanism), Tartu Ülikool.
Piret Arukuusk, magistrikraad, 1987, (juh) , Fosfolipaas A2, Neuraminidaasi ja proteaaside mõju membraanseotud ja solubiliseeritud muskariinsele retseptorile, Tartu Ülikool.
 
 
Teadustöö põhisuunad
ETIS KLASSIFIKAATOR: 1. Bio- ja keskkonnateadused; 1.12. Bio- ja keskkonnateadustega seotud uuringud, näiteks biotehnoloogia, molekulaarbioloogia, rakubioloogia, biofüüsika, majandus- ja tehnoloogiauuringud; CERCS KLASSIFIKAATOR: T360 Biokeemiatehnoloogia

Lõppenud projektid

Juhendatud väitekirjad

Publikatsioonid

Klass
Aasta
Publikatsioon
 
1.1.
2018
Freimann, Krista; Arukuusk, Piret; Kurrikoff, Kaido; Pärnaste, Ly; Raid, Raivo; Piirsoo, Andres; Pooga, Margus; Lange, Ülo (2018). Formulation of stable and homogenous cell penetrating peptide NF55 nanoparticles for efficient gene delivery in vivo. Molecular Therapy – Nucleic Acids, 10, 28−35.10.1016/j.omtn.2017.10.011.
1.1.
2017
Juks, C.; Lorents, A.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2017). Cell-penetrating peptides recruit type A scavenger receptors to the plasma membrane for cellular delivery of nucleic acids. The FASEB Journal, 31 (3), 975−988.10.1096/fj.201600811r.
1.1.
2017
Pärnaste, Ly; Arukuusk, Piret; Langel, Kent; Tenson, Tanel; Langel, Ülo (2017). The formation of nanoparticles between small interfering RNA and amphipathic cell-penetrating peptides. Molecular Therapy – Nucleic Acids, 7, 1−10.10.1016/j.omtn.2017.02.003.
1.1.
2017
Kurrikoff, Kaido; Veiman, Kadi-Liis; Künnapuu, Kadri; Peets, Elin Madli; Lehto, Tõnis; Pärnaste, Ly; Arukuusk, Piret; Langel, Ülo (2017). Effective in vivo gene delivery with reduced toxicity, achieved by charge and fatty acid -modified cell penetrating peptide. Scientific Reports, 7 (1), 17056−17056.10.1038/s41598-017-17316-y.
1.1.
2016
Margus, Helerin; Arukuusk, Piret; Langel, Ülo; Pooga, Margus (2016). Characteristics of Cell-Penetrating Peptide/Nucleic Acid Nanoparticles. Molecular Pharmaceutics, 13 (1), 172−179.10.1021/acs.molpharmaceut.5b00598.
1.1.
2016
Pärnaste, Ly; Arukuusk, Piret; Zagato, Elisa; Braeckmans, Kevin; Langel, Ülo (2016). Methods to follow intracellular trafficking of cell-penetrating peptides. Journal of Drug Targeting, 24 (6), 508−519.10.3109/1061186X.2015.1095194.
1.1.
2016
Pae, J.; Liivamägi, L.; Etverk, I.; Lubenets, D.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2016). Glycosaminoglycans are required for translocation of amphipathic cell-penetrating peptides across membranes. Biochimica et Biophysica Acta - Biomembranes, 1858 (8), 1860−1867.10.1016/j.bbamem.2016.04.010.
1.1.
2016
Freimann, Krista; Arukuusk, Piret; Kurrikoff, Kaido; Vasconcelos, Luís Daniel Ferreira; Veiman, Kadi-Liis; Uusna, Julia; Margus, Helerin; Garcia-Sosa, Alfonso T; Pooga, Margus; Langel, Ülo (2016). Optimization of in vivo DNA delivery with NickFect peptide vectors. Journal of controlled release : official journal of the Controlled Release Society, 241, 135−143.10.1016/j.jconrel.2016.09.022.
1.1.
2016
Kivi, Rait; Solovjova, Karina; Haljasorg, Tõiv; Arukuusk, Piret; Järv, Jaak (2016). Allosteric Effect of Adenosine Triphosphate on Peptide Recognition by 3'5'-Cyclic Adenosine Monophosphate Dependent Protein Kinase Catalytic Subunits. The Protein Journal, 35 (6), 459−466.10.1007/s1093.
1.1.
2015
Arukuusk, P.; Pärnaste, L.; Hällbrink, M.; Langel, Ü. (2015). Pepfects and nickfects for the intracellular delivery of nucleic acids (Methods in Molecular Biology). Methods in molecular biology, 1324, 303−315.10.1007/978-1-4939-2806-4_19.
1.1.
2015
Juks, C.; Padari, K.; Margus, H.; Kriiska, A.; Etverk, I.; Arukuusk, P.; Koppel, K.; Ezzat, K.; Langel, Ü.; Pooga, M. (2015). The role of endocytosis in the uptake and intracellular trafficking of PepFect14-nucleic acid nanocomplexes via class A scavenger receptors. Biochimica et Biophysica Acta - Biomembranes, 1848 (12), 3205−3216.10.1016/j.bbamem.2015.09.019.
1.1.
2014
Vasconcelos, Luis; Madani, Fatemeh; Arukuusk, Piret; Pärnaste, Ly; Gräslund, Astrid, Langel, Ülo (2014). Effects of cargo molecules on membrane perturbation caused by transportan10 based cell-penetrating peptides. Biochimica et Biophysica Acta, 1838 (12), 3118−3129.10.1016/j.bbamem.2014.08.011.
1.1.
2014
Pae, J.; Säälik, P.; Liivamägi, L.; Lubenets, D.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2014). Translocation of cell-penetrating peptides across the plasma membrane is controlled by cholesterol and microenvironment created by membranous proteins. Journal of Controlled Release, 192, 103−113.10.1016/j.jconrel.2014.07.002.
1.1.
2014
Rytkönen, Jussi; Arukuusk, Piret; Xu, Wujun; Kurrikoff, Kaido; Langel, Ülo; Lehto, Vesa-Pekka; Närvänen, Ale (2014). Porous Silicon–Cell Penetrating Peptide Hybrid Nanocarrier for Intracellular Delivery of Oligonucleotides. Molecular Pharmaceutics, 11 (2), 382−390.10.1021/mp4002624.
5.1.
2014
Margus, H.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2014). Morphology of cell-penetrating peptide-nucleic acid nanoparticles and their intracellular trafficking. FEBS JOURNAL, 281, SI: FEBS EMBO 2014, Paris. Wiley-Blackwell, 413−414.
1.1.
2013
Arukuusk, P; Pärnaste, L.; Margus, H.; Eriksson, J.; Vasconcelos, L.;.Padari, K.; Pooga, M.; Langel, Ü. (2013). Differential endosomal pathways for radically modified peptide vectors. Bioconjugate Chemistry, 24 (10), 1721−1732.10.1021/bc4002757.
1.1.
2013
Arukuusk, P.; Pärnaste, L.; Oskolkov, N.; Copolovici, D. M.; Margus, H.; Padari, K.; Möll, K.; Maslovskaja, J.; Tegova, R.; Kivi, G.; Tover, A.; Pooga, M.; Ustav, M.; Langel, Ü. (2013). New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids. Biochimica et Biophysica Acta - Biomembranes, 1828 (5), 1365−1373.10.1016/j.bbamem.2013.01.011.
1.1.
2013
Veiman, K.L.; Mäger, I.; Ezzat, K.; Margus, H.; Lehto, T.; Langel, K.; Kurrikoff, K.; Arukuusk, P.; Suhorutšenko, J.; Padari, K.; Pooga, M.; Lehto, T.; Langel, Ü. (2013). PepFect14 peptide vector for efficient gene delivery in cell cultures. Molecular Pharmaceutics, 10 (1), 199−210.10.1021/mp3003557.
1.1.
2011
Suhorutšenko, J.; Oskolkov, N.; Arukuusk, P.; Kurrikoff, K.; Eriste, E.; Copolovici, D.-M.; Langel, Ü. (2011). Cell-Penetrating Peptides, PepFects, Show No Evidence of Toxicity and Immunogenicity In Vitro and In Vivo. Bioconjugate Chemistry, 22 (11), 2255−2262.dx.doi.org/10.1021/bc200293d.
1.1.
2011
Oskolkov, N.; Arukuusk, P.; Copolovici, D.-M.; Lindberg, S.; Margus, H.; Padari, K.; Pooga, M.; Langel, Ü. (2011). NickFects, Phosphorylated Derivatives of Transportan 10 for Cellular Delivery of Oligonucleotides. International Journal of Peptide Research and Therapeutics, 17 (2), 147−157.10.1007/s10989-011-9252-1.
1.1.
2010
Langel, K; Lindberg, S; Copolovici, D; Arukuusk, P; Sillard, R; Langel, Ü. (2010). Novel fatty acid modifications of transportan 10. International Journal of Peptide Research and Therapeutics, 16 (4), 247−255.10.1007/s10989-010-9224-x.
5.1.
2010
Suhorutsenko, J.; Copolovici, D. M.; Eriste, E.; Oskolkov, N.; Arukuusk, P.; Langel, Ü. (2010). New cell-penetrating peptides induce no inflammatory response in antigen-presenting cell lines. Scandinavian Journal of Immunology, 71: 39th meeting of Scandinavian Society for Immunology jointly with the Baltic Immunologycal Society; Tallinn, Estonia; 2-5.06.2010. Blackwell Publishing Ltd, 508−509. (474-548; 6).10.1111/j.1365-3083.2010.02392.x.
3.2.
1989
Järv, J.; Arukuusk, P.; Kõiv, A.; Rinken, A. (1989). Muscarinic receptor - biomembrane interaction. In: Kotyr ,A.; Skoda, J.; Paces, V.; Kostka, V. (Ed.). Highlights of Modern Biochemistry (1073−1082).. Praha: Zeist.

Tööstusomand

21.02.2020

Piret Arukuusk

https://www.etis.ee/CV/Piret_Arukuusk/eng
piret.arukuusk@ut.ee

Career

Institutions and positions
01.01.2016–...   
University of Tartu, Faculty of Science and Technology, Institute of Technology, Research Fellow in Peptide Chemistry (1,00)
15.11.2013–31.07.2015   
University of Tartu, Faculty of Science and Technology (old), Institute of Technology, University of Tartu, Research Fellow in Molecular Biotechnology (1,00)
01.09.2009–14.11.2013   
University of Tartu, Faculty of Science and Technology (old), Institute of Technology, University of Tartu, Specialist (1,00)
 
 
Education
2011–2013   
Tartu University, PhD student

Qualifications

Academic degrees
Piret Arukuusk, Doctor's Degree, 2013, (sup) Ülo Langel, NickFects - novel cell-penetrating peptides. Design and uptake mechanism, University of Tartu.
Piret Arukuusk, Master's Degree, 1987, (sup) , Fosfolipaas A2, Neuraminidaasi ja proteaaside mõju membraanseotud ja solubiliseeritud muskariinsele retseptorile, University of Tartu.
 
 
Fields of research
ETIS CLASSIFICATION: 1. Biosciences and Environment; 1.12. Biotechnology, Molecular Biology, Cell Biology, Biophysics and Economic and Technological Research relating to Bio- and Environmental Sciences; CERCS CLASSIFICATION: T360 Biochemical technology
 
 

Completed projects

Supervised dissertations

Publications

Category
Year
Publication
 
1.1.
2018
Freimann, Krista; Arukuusk, Piret; Kurrikoff, Kaido; Pärnaste, Ly; Raid, Raivo; Piirsoo, Andres; Pooga, Margus; Lange, Ülo (2018). Formulation of stable and homogenous cell penetrating peptide NF55 nanoparticles for efficient gene delivery in vivo. Molecular Therapy – Nucleic Acids, 10, 28−35.10.1016/j.omtn.2017.10.011.
1.1.
2017
Juks, C.; Lorents, A.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2017). Cell-penetrating peptides recruit type A scavenger receptors to the plasma membrane for cellular delivery of nucleic acids. The FASEB Journal, 31 (3), 975−988.10.1096/fj.201600811r.
1.1.
2017
Pärnaste, Ly; Arukuusk, Piret; Langel, Kent; Tenson, Tanel; Langel, Ülo (2017). The formation of nanoparticles between small interfering RNA and amphipathic cell-penetrating peptides. Molecular Therapy – Nucleic Acids, 7, 1−10.10.1016/j.omtn.2017.02.003.
1.1.
2017
Kurrikoff, Kaido; Veiman, Kadi-Liis; Künnapuu, Kadri; Peets, Elin Madli; Lehto, Tõnis; Pärnaste, Ly; Arukuusk, Piret; Langel, Ülo (2017). Effective in vivo gene delivery with reduced toxicity, achieved by charge and fatty acid -modified cell penetrating peptide. Scientific Reports, 7 (1), 17056−17056.10.1038/s41598-017-17316-y.
1.1.
2016
Margus, Helerin; Arukuusk, Piret; Langel, Ülo; Pooga, Margus (2016). Characteristics of Cell-Penetrating Peptide/Nucleic Acid Nanoparticles. Molecular Pharmaceutics, 13 (1), 172−179.10.1021/acs.molpharmaceut.5b00598.
1.1.
2016
Pärnaste, Ly; Arukuusk, Piret; Zagato, Elisa; Braeckmans, Kevin; Langel, Ülo (2016). Methods to follow intracellular trafficking of cell-penetrating peptides. Journal of Drug Targeting, 24 (6), 508−519.10.3109/1061186X.2015.1095194.
1.1.
2016
Pae, J.; Liivamägi, L.; Etverk, I.; Lubenets, D.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2016). Glycosaminoglycans are required for translocation of amphipathic cell-penetrating peptides across membranes. Biochimica et Biophysica Acta - Biomembranes, 1858 (8), 1860−1867.10.1016/j.bbamem.2016.04.010.
1.1.
2016
Freimann, Krista; Arukuusk, Piret; Kurrikoff, Kaido; Vasconcelos, Luís Daniel Ferreira; Veiman, Kadi-Liis; Uusna, Julia; Margus, Helerin; Garcia-Sosa, Alfonso T; Pooga, Margus; Langel, Ülo (2016). Optimization of in vivo DNA delivery with NickFect peptide vectors. Journal of controlled release : official journal of the Controlled Release Society, 241, 135−143.10.1016/j.jconrel.2016.09.022.
1.1.
2016
Kivi, Rait; Solovjova, Karina; Haljasorg, Tõiv; Arukuusk, Piret; Järv, Jaak (2016). Allosteric Effect of Adenosine Triphosphate on Peptide Recognition by 3'5'-Cyclic Adenosine Monophosphate Dependent Protein Kinase Catalytic Subunits. The Protein Journal, 35 (6), 459−466.10.1007/s1093.
1.1.
2015
Arukuusk, P.; Pärnaste, L.; Hällbrink, M.; Langel, Ü. (2015). Pepfects and nickfects for the intracellular delivery of nucleic acids (Methods in Molecular Biology). Methods in molecular biology, 1324, 303−315.10.1007/978-1-4939-2806-4_19.
1.1.
2015
Juks, C.; Padari, K.; Margus, H.; Kriiska, A.; Etverk, I.; Arukuusk, P.; Koppel, K.; Ezzat, K.; Langel, Ü.; Pooga, M. (2015). The role of endocytosis in the uptake and intracellular trafficking of PepFect14-nucleic acid nanocomplexes via class A scavenger receptors. Biochimica et Biophysica Acta - Biomembranes, 1848 (12), 3205−3216.10.1016/j.bbamem.2015.09.019.
1.1.
2014
Vasconcelos, Luis; Madani, Fatemeh; Arukuusk, Piret; Pärnaste, Ly; Gräslund, Astrid, Langel, Ülo (2014). Effects of cargo molecules on membrane perturbation caused by transportan10 based cell-penetrating peptides. Biochimica et Biophysica Acta, 1838 (12), 3118−3129.10.1016/j.bbamem.2014.08.011.
1.1.
2014
Pae, J.; Säälik, P.; Liivamägi, L.; Lubenets, D.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2014). Translocation of cell-penetrating peptides across the plasma membrane is controlled by cholesterol and microenvironment created by membranous proteins. Journal of Controlled Release, 192, 103−113.10.1016/j.jconrel.2014.07.002.
1.1.
2014
Rytkönen, Jussi; Arukuusk, Piret; Xu, Wujun; Kurrikoff, Kaido; Langel, Ülo; Lehto, Vesa-Pekka; Närvänen, Ale (2014). Porous Silicon–Cell Penetrating Peptide Hybrid Nanocarrier for Intracellular Delivery of Oligonucleotides. Molecular Pharmaceutics, 11 (2), 382−390.10.1021/mp4002624.
5.1.
2014
Margus, H.; Arukuusk, P.; Langel, Ü.; Pooga, M. (2014). Morphology of cell-penetrating peptide-nucleic acid nanoparticles and their intracellular trafficking. FEBS JOURNAL, 281, SI: FEBS EMBO 2014, Paris. Wiley-Blackwell, 413−414.
1.1.
2013
Arukuusk, P; Pärnaste, L.; Margus, H.; Eriksson, J.; Vasconcelos, L.;.Padari, K.; Pooga, M.; Langel, Ü. (2013). Differential endosomal pathways for radically modified peptide vectors. Bioconjugate Chemistry, 24 (10), 1721−1732.10.1021/bc4002757.
1.1.
2013
Arukuusk, P.; Pärnaste, L.; Oskolkov, N.; Copolovici, D. M.; Margus, H.; Padari, K.; Möll, K.; Maslovskaja, J.; Tegova, R.; Kivi, G.; Tover, A.; Pooga, M.; Ustav, M.; Langel, Ü. (2013). New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids. Biochimica et Biophysica Acta - Biomembranes, 1828 (5), 1365−1373.10.1016/j.bbamem.2013.01.011.
1.1.
2013
Veiman, K.L.; Mäger, I.; Ezzat, K.; Margus, H.; Lehto, T.; Langel, K.; Kurrikoff, K.; Arukuusk, P.; Suhorutšenko, J.; Padari, K.; Pooga, M.; Lehto, T.; Langel, Ü. (2013). PepFect14 peptide vector for efficient gene delivery in cell cultures. Molecular Pharmaceutics, 10 (1), 199−210.10.1021/mp3003557.
1.1.
2011
Suhorutšenko, J.; Oskolkov, N.; Arukuusk, P.; Kurrikoff, K.; Eriste, E.; Copolovici, D.-M.; Langel, Ü. (2011). Cell-Penetrating Peptides, PepFects, Show No Evidence of Toxicity and Immunogenicity In Vitro and In Vivo. Bioconjugate Chemistry, 22 (11), 2255−2262.dx.doi.org/10.1021/bc200293d.
1.1.
2011
Oskolkov, N.; Arukuusk, P.; Copolovici, D.-M.; Lindberg, S.; Margus, H.; Padari, K.; Pooga, M.; Langel, Ü. (2011). NickFects, Phosphorylated Derivatives of Transportan 10 for Cellular Delivery of Oligonucleotides. International Journal of Peptide Research and Therapeutics, 17 (2), 147−157.10.1007/s10989-011-9252-1.
1.1.
2010
Langel, K; Lindberg, S; Copolovici, D; Arukuusk, P; Sillard, R; Langel, Ü. (2010). Novel fatty acid modifications of transportan 10. International Journal of Peptide Research and Therapeutics, 16 (4), 247−255.10.1007/s10989-010-9224-x.
5.1.
2010
Suhorutsenko, J.; Copolovici, D. M.; Eriste, E.; Oskolkov, N.; Arukuusk, P.; Langel, Ü. (2010). New cell-penetrating peptides induce no inflammatory response in antigen-presenting cell lines. Scandinavian Journal of Immunology, 71: 39th meeting of Scandinavian Society for Immunology jointly with the Baltic Immunologycal Society; Tallinn, Estonia; 2-5.06.2010. Blackwell Publishing Ltd, 508−509. (474-548; 6).10.1111/j.1365-3083.2010.02392.x.
3.2.
1989
Järv, J.; Arukuusk, P.; Kõiv, A.; Rinken, A. (1989). Muscarinic receptor - biomembrane interaction. In: Kotyr ,A.; Skoda, J.; Paces, V.; Kostka, V. (Ed.). Highlights of Modern Biochemistry (1073−1082).. Praha: Zeist.

Industrial property

21.02.2020
Detailne otsing
  • Leitud 6 kirjet
ProgrammLiikNumberNimiProjekti algusProjekti lõppVastutav täitjaAsutusFinantseerijapoolne rahastamine
MUUSLOTI11182T (3.2.0701.11-0016)Hepatiit C viiruse vastaste ravimikandidaatide väljatöötamise platvorm01.01.201231.12.2013Andres MeritsTartu Ülikool; Tartu Ülikool, Loodus- ja tehnoloogiateaduskond872 230,20 EUR
SFSF0180027s08Koespetsiifilised transportvektorid01.01.200831.12.2013Ülo LangelTartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut600 272,61 EUR
TKSLTTI16143T (TK143)Molekulaarse Rakutehnoloogia Tippkeskus (TK143)01.01.201601.03.2023Tanel TensonTartu Ülikool, Loodus- ja täppisteaduste valdkond, tehnoloogiainstituut3 917 568,00 EUR
2.1.1.LLTTI19567 Rakku penetreeruvate peptiidide kasutamine transfektsioonireagendina01.10.201930.09.2020Kaido KurrikoffTartu Ülikool, Loodus- ja täppisteaduste valdkond, tehnoloogiainstituut160 033,90 EUR
IUTIUT20-26Trans-barrier targeting01.01.201431.12.2019Ülo LangelTartu Ülikool, Loodus- ja täppisteaduste valdkond, tehnoloogiainstituut889 200,00 EUR
ETFETF9438Uued meetodid therapeutikumide raku spetsiifiliseks sisestamiseks01.01.201201.12.2015Julia UusnaTartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Tehnoloogiainstituut46 440,00 EUR