"Sihtfinantseerimine" projekt SF0180045s08
SF0180045s08 "Fotosünteesi regulatsioon seoses sekundaarse metabolismiga lämmastiku ja isopreeni näitel (1.01.2008−31.12.2013)", Agu Laisk, Tartu Ülikool, Bioloogia-geograafiateaduskond, Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut, Tartu Ülikool, Loodus- ja tehnoloogiateaduskond.
SF0180045s08
Fotosünteesi regulatsioon seoses sekundaarse metabolismiga lämmastiku ja isopreeni näitel
Control of photosynthesis in relation to secondary metabolism on examples of nitrogen and isoprene
1.01.2008
31.12.2013
Sihtfinantseerimine
ValdkondAlamvaldkondCERCS erialaFrascati Manual’i erialaProtsent
1. Bio- ja keskkonnateadused1.12. Bio- ja keskkonnateadustega seotud uuringud, näiteks biotehnoloogia, molekulaarbioloogia, rakubioloogia, biofüüsika, majandus- ja tehnoloogiauuringudP330 Bioenergeetika 1.5. Bioteadused (bioloogia, botaanika, bakterioloogia, mikrobioloogia, zooloogia, entomoloogia, geneetika, biokeemia, biofüüsika jt100,0
AsutusRollPeriood
Tartu Ülikool, Bioloogia-geograafiateaduskondkoordinaator01.01.2008−31.08.2008
Tartu Ülikool, Loodus- ja tehnoloogiateaduskond, Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituutkoordinaator01.01.2010−31.12.2013
Tartu Ülikool, Loodus- ja tehnoloogiateaduskondkoordinaator01.09.2008−31.12.2009
PerioodSumma
01.01.2008−31.12.20081 890 000,00 EEK (120 793,02 EUR)
01.01.2009−31.12.20091 734 336,00 EEK (110 844,27 EUR)
01.01.2010−31.12.20101 636 200,00 EEK (104 572,24 EUR)
01.01.2011−31.12.2011104 570,00 EUR
01.01.2012−31.12.2012104 570,00 EUR
01.01.2013−31.12.2013104 570,00 EUR
649 919,53 EUR

Meie pikaajalise fundamentaaluuringu eesmärk on mõista taimelehe fotosünteesi piiravaid ja reguleerivaid tegureid. Tööhüpotees on mehhanitsistlik seos lämmastiku ja süsiniku metabolismi vahel, mis kindlustab taime kasvuks optimaalse valgu ja süsivesikute suhte. Hüpoteesi aluseks on varasemad tulemused, et vastupidiselt levinud arvamusele, tsükliline elektrontransport ei kanna prootoneid, vaid on dissipatiivse iseloomuga. Fotosünteesis reguleerib ATP/NADPH suhet peamiselt lämmastikku taandav “alternatiivne” elektrontransport, mille kaudu lämmastiku metabolism kontrollibki fotosünteesi. Katselised andmed põhinevad peamiselt O2/CO2 suhte mõõtmisel erineval määral tärklist sünteesivatel ja lämmastikku taandavatel lehtedel ja nende analüüsil fotosünteesi matemaatilise mudeli abil. Arendatakse välja ka laboratoorium isopreeni ja fotosünteesi mõõtmiseks. Hüpotees on, et isopreen eraldub sünteesiraja jääkproduktina juhul kui raja vaheprodukte ei tarbita orgaaniliselt vajalikeks sünteesideks.
This research project is to understand rate-controlling factors of leaf photosynthesis. Working hypothesis is the mechanistic link between carbon and nitrogen metabolisms ensuring the optimal ratio of protein and carbohydrate. The hypothesis is based on our results that cyclic electron transport is a dissipative process, uncoupled from proton transport. The ATP/NADPH ratio is adjusted mainly by the “alternative” electron flow for N reduction, by which nitrogen metabolism is controlling photosynthesis. Experimentally, mainly O2/CO2 exchange ratio will be measured on plants having different starch synthesis and nitrate reduction rates, and on the analysis of these data with the mathematical model of photosynthesis. Also, a laboratory for the measurements of isoprene during photosynthesis will be developed. It is hypothesized that isoprene evolves as a residual end-product of the pathway when the intermediates are not completely consumed for the build-up of the photosynthetic machinery.

Vastutav täitja (1)

IsikKraadTöökoht ja ametCVOsalemise periood
Agu LaiskdoktorikraadEST / ENG01.01.2008−31.12.2013

Põhitäitjad (6)

IsikKraadTöökoht ja ametCVOsalemise periood
Irina Bichelemagistrikraad (teaduskraad)EST / ENG01.01.2008−31.12.2013
Hillar Eichelmannmagistrikraad (teaduskraad)EST / ENG01.01.2008−31.12.2013
Vello OjadoktorikraadEST / ENG01.01.2008−31.12.2013
Evi PadudoktorikraadEST / ENG01.01.2008−31.12.2013
Bakhtier RasulovdoktorikraadEST / ENG01.04.2008−31.12.2013
Eero TaltsdoktorikraadEST / ENG01.01.2010−31.12.2012

Täitjad (1)

IsikKraadTöökoht ja ametCVOsalemise periood
Eero TaltsdoktorikraadEST / ENG01.01.2008−31.12.2012

Projektiga seotud tööjõud (1)

IsikKraadAsutusAmetKoormusKoormus väljaspoolOsalemise periood
ProjektisKoormus väljaspool
Heikko Rämmajuhtiv spetsialist101.01.2008−31.12.2013
Publikatsioonid
Publikatsioonid
Oja, V.; Eichelmann, H.; Anijalg, A.; Rämma, H.; Laisk, A. (2010). Equilibrium or disequilibrium? A dual-wavelength investigation of photosystem I donors. Photosynthesis Research, 103 (3), 153−166.10.1007/s11120-010-9534-z.
Laisk, A.; Talts, E.; Oja, V.; Eichelmann, H.; Peterson, R. (2010). Fast cyclic electron transport around photosystem I in leaves under far-red light: a proton-uncoupled pathway? Photosynthesis Research, 103, 79−95.10.1007/s11120-009-9513-4.
Rasulov, B.; Copolovici, L.; Laisk, A.; Niinemets, Ü. (2009). Postillumination isoprene emission: in vivo measurements of dimethylallylpyrophosphate pool size and isoprene synthase kinetics in aspen leaves. Plant Physiology, 149 (3), 1609−1618.10.1104/pp.108.133512.
Rasulov, Bahtijor; Hüve, Katja; Välbe, Mikk; Laisk, Agu; Niinemets, Ülo (2009). Evidence that light, carbon dioxide, and oxygen dependencies of leaf isoprene emission are driven by energy status in hybrid aspen. Plant Physiology, 151, 448−460.
Eichelmann, H.; Talts, E.; Oja, V.; Padu, E.; Laisk, A. (2009). Rubisco in planta kcat is regulated in balance with photosynthetic electron transport. Journal of Experimental Botany, 60 (14), 4077−4088.10.1093/jxb/erp242.
Laisk, A.; Eichelmann, H.; Oja, V. (2009). Leaf C3 photosynthesis in silico. Integrated carbon/nitrogen metabolism. In: Laisk, Agu; Nedbal, Ladislav; Govindjee (Ed.). Photosynthesis in silico. Understanding Complexity from Molecules to Ecosystems (295−322).. Dordrecht, The Netherlands: Springer. (Advances in Photosynthesis and Respiration; 29).
Laisk, A.; Edwards, G. (2009). Leaf C4 photosynthesis in silico: The CO2 concentrating mechanism. In: Laisk, Agu; Nedbal, Ladislav; Govindjee (Ed.). Photosynthesis in silico. Understanding Complexity from Molecules to Ecosystems (323−348).. Dordrecht, The Netherlands: Springer. (Advances in Photosynthesis and Respiration; 29).
Laisk, A; Nedbal, L; Govindjee, G. (2009). Photosynthesis in silico. Understanding Complexity from Molecules to Ecosystems. Dordrecht, The Netherlands: Springer.
Laisk, A.; Oja, V.; Eichelmann, H. (2008). Alternative and cyclic electron flow: rate and role in potato leaves. In: Allen, J.F.; Gantt, E.; Golbeck, J.H.; Osmond, B. (Ed.). Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis (913−916).. Springer.
Talts, E.; Oja, V.; Rämma, H.; Rasulov, B.; Anijalg, A.; Laisk, A. (2008). Dark inactivation of ferredoxin-NADP reductase and cyclic electron transport under far-red light in sunflower leaves. In: Allen, J.F.; Gantt, E.; Golbeck, J.H.; Osmond, B. (687−690).. Springer.
Oja, V.; Eichelmann, H.; Laisk, A. (2008). Equilibrium or disequilibrium? A dual-wavelength investigation of photosystem I donors. In: Allen, J.F.; Gantt, E.; Golbeck, J.H.; Osmond, B. (Ed.). Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis (687−690).. Springer.
Eichelmann, H.; Talts, E.; Oja, V.; Rasulov, B.; Padu, E.; Laisk, A. (2008). Rubisco activity is is related to photosystem I in leaves. In: Allen, J.F.; Gantt, E.; Golbeck, J.H.; Osmond, B. (Ed.). Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis (853−856).. Springer.
Rasulov, B.; Hüve, K.; Laisk, A.; Niinemets, Ü. (2011). Induction of a longer term component of isoprene release in darkened aspen leaves: origin and regulation under different environmental conditions. Plant Physiology, 156 (2), 816−831.
Laisk, Agu (2011). Usun, et mõistan. Parmasto, Erast; Laisk, Agu; Kaljo, Dimitri (Toim.). Teadusmõte Eestis (6). Elu- ja maateadused (57−71).. Tallinn: Eesti Teaduste Akadeemia.
Rasulov, B.; Hüve, K.; Bichele, I.; Laisk, A.; Niinemets, Ü. (2010). Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis. Plant Physiology, 154 (3), 1558−1570.
Hüve, K.; Bichele, I.; Rasulov, B.; Niinemets, Ü. (2011). When it is too hot for photosynthesis: heat-induced instability of photosynthesis in relation to respiratory burst, cell permeability changes and H2O2 formation. Plant, Cell and Environment, 34, 113−126.
Laisk, Agu;Oja, Vello; Eichelmann, Hillar; Dall'Osto, Luca (2014). Action spectra of photosystems II and I and quantum yield of photosynthesis in leaves in State 1. Biochimica et Biophysica Acta-Bioenergetics, 1837 (2), 315−325.10.1016/j.bbabio.2013.12.001.
Rasulov, Bahtijor; Bichele, Irina; Laisk, Agu; Niinemets, Ülo (2014). Competition between isoprene emission and pigment synthesis during leaf development in aspen. Plant, Cell and Environment, 37 (3), 724−741.10.1111/pce.12190.
Laisk, Agu; Oja, Vello (2013). Thermal phase and excitonic connectivity in fluorescence induction. Photosynthesis Research, 117, 431−448.10.1007/s11120-013-9915-1.
Laisk, Agu; Oja, Vello; Eichelmann, Hillar (2012). Oxygen eveolution and chlorophyll fluorescence from multiple turnover light pulses: charge recombination in photosystem II in sunflower leaves. Photosynthesis Research, 113, 145−155.10.1007/s11120-012-9751-8.
Oja, Vello; Laisk, Agu (2012). Photosystem II antennae are not energetically connected: evidence based on flash-induced O2 evolution and chlorophyll fluorescence in sunflower leaves. Photosynthesis Research, 114 (1), 15−28.10.1007/s11120-012-9775-0.
Oja, Vello; Eichelmann, Hillar; Laisk, Agu (2011). Oxygen evolution from single- and multiple-turnover light pulses: temporal kinetics of electron transport through PSII in sunflower leaves. Photosynthesis Research, 110 (2), 99−109.10.1007/s11120-011-9702-9.
Eichelmann, H.; Oja, V.; Peterson, R.; Laisk, A. (2011). The rate of nitrite reduction in leaves as indicated by O2 and CO2 exchange during photosynthesis. Journal of Experimental Botany, 62, 2205−2215.
Oja, V.; Eichelmann, H.; Laisk, A. (2011). The size of the lumenal proton pool in leaves during induction and steady-state photosynthesis. Photosynthesis Research, 110 (2), 1−16.DOI10.1007/s11120-011-9697-2.