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"Muu" projekt KBFI,MG-2
KBFI,MG-2 (7/2005, 7/2006) "Rekombinantsed luminestseeruvad biosensorid - keskkonnarealistlikudmudelorganismid toksiliste raskemetallide biokättesaadavuse määramiseks (1.01.2005−31.12.2006)", Anne Kahru, Keemilise ja Bioloogilise Füüsika Instituut.
7/2005, 7/2006
KBFI,MG-2
Rekombinantsed luminestseeruvad biosensorid - keskkonnarealistlikudmudelorganismid toksiliste raskemetallide biokättesaadavuse määramiseks
Bioavailability of toxic heavy metals: recombinant luminescent biosensors and environmental reality
Bioavailability of toxic heavy metals: recombinant luminescent biosensors and environmental reality
1.01.2005
31.12.2006
Teadus- ja arendusprojekt
Muu
ValdkondAlamvaldkondCERCS erialaFrascati Manual’i erialaProtsent
1. Bio- ja keskkonnateadused1.2. MikrobioloogiaB230 Mikrobioloogia, bakterioloogia, viroloogia, mükoloogia 1.5. Bioteadused (bioloogia, botaanika, bakterioloogia, mikrobioloogia, zooloogia, entomoloogia, geneetika, biokeemia, biofüüsika jt70,0
4. Loodusteadused ja tehnika4.16. Biotehnoloogia (loodusteadused ja tehnika)T360 Biokeemiatehnoloogia 2.3. Teised tehnika- ja inseneriteadused (keemiatehnika, lennundustehnika, mehaanika, metallurgia, materjaliteadus ning teised seotud erialad: puidutehnoloogia, geodeesia, tööstuskeemia, toiduainete tehnoloogia, süsteemianalüüs, metallurgia, mäendus, tekstiilitehnoloogia ja teised seotud teadused).30,0
AsutusRollPeriood
Keemilise ja Bioloogilise Füüsika Instituutkoordinaator01.01.2005−31.12.2006
AsutusRiikTüüp
Eesti-Prantsuse teadus- ja tehnoloogiaalane koostööprogramm PARROT (Eesti Teadusfondi kaudu)
PerioodSumma
01.01.2005−31.12.200625 200,00 EEK (1 610,57 EUR)
1 610,57 EUR
Eesti-Prantsuse teadus- ja tehnoloogiaalane koostööprogramm PARROT (Eesti Teadusfondi kaudu)

Heavy metals are group of known environmental pollutants that include very toxic elements like mercury, cadmium and lead, which production around the world steadily increases. Due to the badly managed emission control and sorptive capacity of soils and sediments, they continuously accumulate in the environment producing extremely polluted habitats. However, it is known that the organisms living in these polluted environments are affected only by a relatively small fraction of these accumulated metals. This hazardous or bioavailable fraction of metals in soils could be determined only by using bioassays with soil-specific organisms e.g., earthworms, algae, protozoa or bacteria. In addition to the naturally occuring bacteria it is possible to use also man-modified bacteria, which report on the presence of heavy metals by increasing of e.g., luminescence. These sensor bacteria – heroes of that project - offer totally new possibilities for the specific determination of bioavailable metals as they emit light only while exposed to certain heavy metal. The aim of this project was to evaluate the hazard of heavy metal polluted soils using different ecotoxicological tests and to construct and modify sensor bacteria in order to increase their sensitivity and specificity. Our results showed that even in very polluted soils sampled around the metal smelters in Northern France, the bioavailability of heavy metals was very low – acute toxic effects were detected only in case of few samples and organisms. Heavy metal sensor bacteria, however, showed that the metals entered their cells and triggered the „defence” mechanisms of these bacteria against toxic metals. As the sensitivity of the existing sensor bacteria was not enough to detect the bioavailable concentrations of metals in a number of samples, bacteria with increased sensing capabilities were constructed. For that the genes regulating the bacterial intracellular concentration of heavy metals were modified. The results showed that indeed these tailored modifications enabled to sensitize the bacteria for heavy metals opening the possibility for development of hypersensitive test bacteria for environmental monitoring.
Heavy metals are group of known environmental pollutants that include very toxic elements like mercury, cadmium and lead, which production around the world steadily increases. Due to the badly managed emission control and sorptive capacity of soils and sediments, they continuously accumulate in the environment producing extremely polluted habitats. However, it is known that the organisms living in these polluted environments are affected only by a relatively small fraction of these accumulated metals. This hazardous or bioavailable fraction of metals in soils could be determined only by using bioassays with soil-specific organisms e.g., earthworms, algae, protozoa or bacteria. In addition to the naturally occuring bacteria it is possible to use also man-modified bacteria, which report on the presence of heavy metals by increasing of e.g., luminescence. These sensor bacteria – heroes of that project - offer totally new possibilities for the specific determination of bioavailable metals as they emit light only while exposed to certain heavy metal. The aim of this project was to evaluate the hazard of heavy metal polluted soils using different ecotoxicological tests and to construct and modify sensor bacteria in order to increase their sensitivity and specificity. Our results showed that even in very polluted soils sampled around the metal smelters in Northern France, the bioavailability of heavy metals was very low – acute toxic effects were detected only in case of few samples and organisms. Heavy metal sensor bacteria, however, showed that the metals entered their cells and triggered the „defence” mechanisms of these bacteria against toxic metals. As the sensitivity of the existing sensor bacteria was not enough to detect the bioavailable concentrations of metals in a number of samples, bacteria with increased sensing capabilities were constructed. For that the genes regulating the bacterial intracellular concentration of heavy metals were modified. The results showed that indeed these tailored modifications enabled to sensitize the bacteria for heavy metals opening the possibility for development of hypersensitive test bacteria for environmental monitoring.