"Personal Research Funding" project PUT34
PUT34 "Enhancing Ionization Efficiecy in ESI by a Novel Nebulizer Design (1.01.2013−31.12.2016)", Anneli Kruve, University of Tartu, Faculty of Science and Technology, Institute of Chemistry.
PUT34
Ionisatsiooniefektiivsuse parendamine ESI ioonallikas kasutades uudset pihustit
Enhancing Ionization Efficiecy in ESI by a Novel Nebulizer Design
1.01.2013
31.12.2016
Personal Research Funding
Start-up project
Field of researchSubfieldCERCS specialtyFrascati Manual specialtyPercent
4. Natural Sciences and Engineering4.11. Chemistry and Chemical TechnologyP300 Analytical chemistry 1.3. Chemical sciences (chemistry, other allied subjects)100,0
PeriodSum
01.01.2013−31.12.201349 200,00 EUR
01.01.2014−31.12.201449 200,00 EUR
01.01.2015−31.12.201549 200,00 EUR
01.01.2016−31.12.201649 200,00 EUR
196 800,00 EUR

LC/MS leiab väga laialdast rakendust alustades toiduohutuse tagamisest lõpetades ravimite määramisega patsientide veres. Nende analüüsitulemuste baasil tehakse olulisi otsuseid nii meditsiinis, keskkonnakaitses kui ka majanduses. ESI on peamine LC/MS analüüsil kasutatav ionisatsioonimeetod tänu universaalsusele ja kasutuslihtsusele. Kahjuks esinevad ESI allikas ionisatsiooni maha suruvad maatriksefektid, mis vähendavad analüüsi tundlikkust ja kõrgendavad määramispiire ning suurendavad valede otsuste tegemise tõenäosust. Seda probleemi pole õnnestunud lahendada ei maatriksefektide arvessevõtmisega ega ka vähendamisega. Meie eesmärgiks on parandada ionisatsiooniefektiivsust läbi parema pihustuse ESI allikas lisades ESI pihustile täiendava pihustusgaasi kapillaari. Sellise kapillaari lisamise idee kohta on esitatud patenditaotlus ning eelkatsed lubavad loota maatriksefektide ja määramispiiride olulist alanemist ning märgatavat tundlikkuse kasvu.
LC/MS is extensively used in applications ranging from food safety to monitoring pharmaceuticals in blood. Critical decisions - medical, environmental, economical - are based on the results of these analyses. ESI is the main ion source in LC/MS due to its universality and robustness. Still ESI suffers from matrix effects, which lead to increased LoD and decreased sensitivity - increasing the risk of wrong decisions. Recently novel ion sources have been designed but none of them has the universality of ESI. Trying to account for or reduce matrix effects has still not been able to eliminate the problem. We propose a novel concept for increasing ionization efficiency by more efficient nebulization in the ESI source by introducing an additional nebulizer capillary. Experiments with a prototype (patent pending) show that this will strongly reduce matrix effects and therefore guarantee lower LoD and higher sensitivity - so badly needed in medical as well and environmental applications.

Principal investigator (1)

PersonDegreeInstitution and occupationCVPeriod
Anneli Kruve-ViilDoctor's DegreeEST / ENG01.01.2013−31.12.2016

Other research staff (5)

PersonDegreeInstitution and occupationCVPeriod
Kristo KleemannMaster's DiplomaEST / ENG01.01.2013−30.09.2013
Asko LaanisteDoctor's DegreeEST / ENG01.01.2013−31.12.2016
Jaanus LiigandMaster's DiplomaEST / ENG01.03.2013−31.12.2016
Piia LiigandMaster's DiplomaEST / ENG01.09.2013−31.12.2016
Vahur TossMaster's DiplomaEST / ENG01.01.2013−30.09.2013
Projects
Project
3.2.1201.13-0020; "Tarkvara arendamine kromatograafiliste analüüsimeetodite valideerimiseks"; Koit Herodes;
IUT20-14; "Superhapete ja superaluste keemia ja selle kõrgtehnoloogilised rakendused"; Ivo Leito;
Publications
Publications
Kruve, Anneli; Leito, Ivo (2013). Comparison of different methods aiming to account for/overcome matrix effects in LC/ESI/MS on the example of pesticide analyses. Analytical methods, 5 (12), 3035−3044.10.1039/C3AY26551J.
Kruve, Anneli; Kaupmees, Karl; Liigand, Jaanus; Oss, Merit; Leito, Ivo (2013). Sodium adduct formation efficiency in ESI source. Journal of Mass Spectrometry, 48 (6), 695−702.10.1002/jms.3218.
Kruve, Anneli; Kaupmees, Karl; Liigand, Jaanus; Leito, Ivo (2014). Negative Electrospray Ionization via Deprotonation: Predicting the Ionization Efficiency. Analytical Chemistry, 86 (10), 4822−4830.10.1021/ac404066v.
Liigand, J.; Kruve, A.; Leito, I.; Girod, M.; Antoine, R. (2014). Effect of Mobile Phase on Electrospray Ionization Efficiency. Journal of the American Society for Mass Spectrometry, 25 (11), 1853−1861.10.1007/s13361-014-0969-x.
Kruve, Anneli; Rebane, Riin; Kipper, Karin; Oldekop, Maarja-Liisa; Evard, Hanno; Herodes, Koit; Ravio, Pekka; Leito, Ivo. (2015). Tutorial review on validation of liquid chromatography–mass spectrometry methods: Part I. Analytica Chimica Acta, 870, 29−44.10.1016/j.aca.2015.02.017.
Kruve, Anneli; Rebane, Riin; Kipper, Karin; Oldekop, Maarja-Liisa; Evard, Hanno; Herodes, Koit; Ravio, Pekka; Leito, Ivo (2015). Tutorial review on validation of liquid chromatography–mass spectrometry methods: Part II. Analytica Chimica Acta, 870, 8−28.10.1016/j.aca.2015.02.016.
Evard, Hanno; Kruve, Anneli; Lõhmus, Rünno; Leito, Ivo. (2015). Paper spray ionization mass spectrometry: Study of a method for fast-screening analysis of pesticides in fruits and vegetables. Journal of Food Composition and Analysis, 41, 221−225.10.1016/j.jfca.2015.01.010.
Liigand, Jaanus; Kruve, Anneli; Liigand, Piia; Laaniste, Asko; Girod, Marion; Antoine, Rodolphe; Leito, Ivo (2015). Transferability of the Electrospray Ionization Efficiency Scale between Different Instruments. Journal of American Society for Mass Spectrometry, x−x [forthcoming].
-