"Personal Research Funding" project PUT1534
PUT1534 "Structural Biology of Viscous Systems (1.01.2017−31.12.2020)", Ago Samoson, Tallinn University of Technology , School of Information Technologies, Department of Health Technologies.
PUT1534
Viskoossete süsteemide struktuuribioloogia
Structural Biology of Viscous Systems
1.01.2017
31.12.2020
R&D project
Personal Research Funding
Exploratory project
ETIS classificationSubfieldCERCS classificationFrascati Manual classificationPercent
4. Natural Sciences and Engineering4.10. Physics and Technical PhysicsP260 Condensed matter: electronic structure, electrical, magnetic and optical properties, supraconductors, magnetic resonance, relaxation, spectroscopy1.2. Physical sciences (astronomy and space sciences, physics, other allied subjects)60,0
4. Natural Sciences and Engineering4.16. Biotechnology relating to Natural Sciences and EngineeringT490 Biotechnology 2.3. Other engineering sciences (such as chemical, aeronautical and space, mechanical, metallurgical and materials engineering, and their specialised subdivisions forest products applied sciences such as geodesy, industrial chemistry, etc. the science and technology of food production specialised technologies of interdisciplinary fields, e.g. systems analysis, metallurgy, mining, textile technology and other allied subjects)20,0
4. Natural Sciences and Engineering4.10. Physics and Technical PhysicsP180 Metrology, physical instrumentation 1.2. Physical sciences (astronomy and space sciences, physics, other allied subjects)20,0
PeriodSum
01.01.2017−31.12.201761 200,00 EUR
01.01.2018−31.12.201861 200,00 EUR
01.01.2019−31.12.201961 200,00 EUR
183 600,00 EUR

Käesolevas taotluses käsitletakse tehnoloogilisi läbimurdeid ja rakendusi viskoossetes elussüsteemides. Struktuuribioloogilstes rakendustes on ultrakiire maagilise nurga all pööritamine (MAS) võtmeküsimuseks spektraalse lahutuse ja tundlikkuse tõstmisel, kuna avaneb võimalus üle minna 1H detekteerimisele. Antud hetkel on maailma tipptulemuseks meie poolt(Samoson et.al.) saavutatud maagilise pööritamise sagedus 130 kHz ning käesolevas projektis on seatud eesmärgiks jõuda 200 kHz-ni. MAS tehnoloogia arenduste rakenduslike eksperimentidena on planeeritud MAS NMR uute võimaluste demonstreerimine fibrillide, membraanide, in-cell ja in-situ fermentatsiooni katsetes. Kuna Alzheimeri tõbi on saavutamas üha olulisemat elustiili ja ka majandust mõjutavat rolli Lääne ühiskonnas, siis seame üheks eesmärgiks uurida Ayurveda meditsiinist tuntud ravimtaimede ekstraktide kasutamist suhteliselt lihtsa kuid samas mõjusa lahendusena fibrillide tekke takistamiseks närvirakkudes.
Viscous Live Science interests are considered in this project. For structural biology in solid state nuclear magnetic resonance magic angle spinning (MAS) speed is essential for a qualitative breakthrough, since 1H detection becomes feasible allowing for two orders of magnitude S/N improvement We already achieved sub-nanomole level, which predicts a huge impact on how structural biology may be carried out e.g. on membranes and other large or poorly soluble complexes, like fibrils. We achieved 130 and shall work for 200 kHz MAS. Cutting edge in-cell in situ MAS experiments are considered for gaining insight into fermentation processes optimizing metabolomics. Since brain functioning is very important issue and Alzheimer becomes a central live style and cost important issue for Western Societies, we target on simple but high impact solutions for plaque forming prevention in nervous cells with plant extracts motivated from Ayurvedic medicine.